DNA Fragmentation Assay.

Clonal Aedes albopictus C6/36 cells transformed with the anti-CHIKV, anti-DENV, anti-CHIKV/DENV or anti-DENV/CHIKV dual targeting antiviral intron constructs indicated were each challenged with each arbovirus indicated. Following virus challenge cells were pelleted, lysed, and analyzed as described in Materials and Methods. DNA fragmentation results obtained following clonal C6/36 cells challenged with A. CHIKV or B. DENV-2 are shown. See S1 Fig through S5 Fig for DNA fragmentation assays performed following challenge with DENV-1, DENV-3, DENV-4, or DNA fragmentation assays performed on uninfected clonal cells expressing anti-CHIKV, anti-DENV or anti-CHIKV/DENV dual targeting antiviral intron constructs. Numbered lanes indicate clonal cell populations expressing single or dual virus targeting intron constructs. Commercial DNA ladder served as size standards for each gel. Size of DNA ladder is indicated on the right of each gel. I = Wt C6/36 mosquito cells infected with virus indicated; U = uninfected; Wt C6/36 mosquito cells; ΔC/Dv1 = ΔCHIKV/DENVv1; ΔD/Cv1 = ΔDENV/CHIKVv1; ΔC/Dv2 = ΔCHIKV/DENVv2; ΔD/Cv2 = ΔDENV/CHIKVv2.