Supplementary methods from Caught in the act: the invasion of a viral vector changes viral prevalence and titre in native honeybees and bumblebees

Supplementary methods including details on bee sampling, PCR used to distinguish Bombus terrestris from B. lucorum and qPCR methods for virus quantification. The supplementary methods also include two tables with all primers that have been used in this study.

本补充方法涵盖蜂类采样的详细步骤、区分地熊蜂（Bombus terrestris）与陆熊蜂（Bombus lucorum）的聚合酶链式反应（Polymerase Chain Reaction, PCR）方案，以及用于病毒定量的实时定量聚合酶链式反应（quantitative Polymerase Chain Reaction, qPCR）实验流程。此外，本补充方法还附有两张附表，汇总了本研究所用的全部引物。