Supplemental Data - Comprehensive profiling of Small RNAs in human embryo-conditioned culture media by improved sequencing and quantitative PCR methods

Supplemental Table 1: Alignment matrix detailing non-coding RNA mapping frequencies (percent total reads) for each sample.<br>Supplemental Table 2: Alignment matrix detailing non-coding RNA mapping frequencies (total raw reads) for each sample.Supplemental Table 3: Normalized read counts for annotated non-coding RNA, excluding no-embryo control samples.Supplemental Table 4: Sequencing spike-in sequences, concentrations, and total input.<br>Supplemental Figure 1: Principal component analysis with control samples excluded. ECCM samples cluster by pool size with single drop pools forming a separate cluster, suggesting that our limit of detection is 3 ECCM drops.<br>Supplemental Figure 2: Spike-in sequencing metrics. The number of molecules of spike-in RNA added to a pool of 3 drops (left y-axis) compared to the measured level of spike-in sequences in reads per million (RPM; right y-axis), totaled across all samples. Per-sample measurements are available in Supplementary Table 4.<br>

补充表1：比对矩阵，详细阐明了各样本的非编码RNA（non-coding RNA）比对频率（总读段占比百分比）。
补充表2：比对矩阵，详细阐明了各样本的非编码RNA比对频率（总原始读段数）。
补充表3：注释后非编码RNA的标准化读段计数，不含无胚胎对照样本。
补充表4：测序内参序列（spike-in）、浓度及总投入量。
补充图1：排除对照样本后的主成分分析（Principal Component Analysis）结果。ECCM样本按混合池大小聚类，单液滴混合池自成一簇，表明本研究的检测限为3个ECCM液滴。
补充图2：内参测序统计量。将添加至3个液滴混合池的内参RNA分子数（左侧纵轴），与所有样本合计的每百万读段数（reads per million, RPM）测得的内参序列水平（右侧纵轴）进行对比。单样本详细数据见补充表4。