Next Generation Sequencing Facilitates Quantitative Analysis of control, NDEA, NDEA + CFT-1 and NDEA+FYT Transcriptomes

Conclusions: Our study represents the first detailed analysis of retinal transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.结论：本研究为首项借助RNA测序（RNA-seq）技术完成的视网膜转录组详细分析，且设置了生物学重复样本。本文报道的优化数据分析流程，可为基因表达谱的比较研究提供标准化分析框架。研究结果表明，下一代测序（Next-Generation Sequencing, NGS）能够对细胞或组织内的mRNA含量实现全面且更为精准的定量与定性评估。综上，基于RNA测序的转录组表征工作可加速遗传网络分析，并助力解析复杂的生物学功能。