RNA-seq of pabpn1l ko and wt female mice oocytes and zygotes

After the library was qualified, the library was pooled according to the effective concentration and the demand of target offline data, and was sequenced by Illumina platform. Since PABPN1L can bind the poly(A) tail, we propose that it participates in the process of post-transcriptional regulation and degradation of maternal mRNA. We investigated the mRNA changes in GV oocytes, MII oocytes, and fertilized eggs using RNA-seq. GV oocytes, MII oocytes and zygotes were collected and mixed from three mice of each genotypes separately.文库质检合格后，依据有效浓度与目标测序数据需求混合文库，并依托Illumina测序平台完成测序。鉴于PABPN1L能够结合poly(A)尾，我们推测其参与母源mRNA的转录后调控与降解过程。本研究采用RNA测序（RNA-seq）技术，对GV卵母细胞（GV oocytes）、MII卵母细胞（MII oocytes）及受精卵的mRNA表达变化进行了分析。本研究分别从每种基因型的三只小鼠体内收集GV卵母细胞、MII卵母细胞与受精卵，并分别进行混合。