Data for: Effective population size mediates the impact of pollination services on pollen limitation

Inadequate pollen receipt limits flowering plant reproduction worldwide. Ecological causes of pollen limitation (‘PL’), like pollinator scarcity and low plant abundance, have been a primary focus of research. The genetic diversity of plant populations could impact both quantity and quality components of PL in concert with ecological factors, yet empirical examples are lacking. We evaluated joint effects of ecological factors (flower abundance, pollinator visitation) and genetic effective population size (NE) on PL across 13 populations of a common herb. We used a histological approach with 5504 styles from 1137 flowers to separate quantity and quality components of PL, and link these to reproductive output. NE and pollinator visitation interacted to shape PL, but NE had stronger direct effects. Effectively smaller populations experienced stronger quantity PL, and controlled crosses in a pollinator-free environment revealed that pollen quantity was an intrinsic population-level attribute..., Datasets include field-collected data in natural plant populations on pollinator visitation rates and floral density, data on pollen-pistil interactions from field-collected flowers, population genomic metrics dervied from RAD-Seq data (previously published), and crosses performed in greenhouse conditions. , R v. 4.2.1, # Data for: Effective population size mediates the impact of pollination services on pollen limitation

Datasets include pollinator visitation data from natural populations, histological data on pollen-pistil interactions from flowers collected in the field and population attribute data (effective population size, flower abundance, elevation etc.). R code for analyses in the manuscript are also provided.

## Description of the data and file structure

In this study we related pollen limitation with pollinator visitation, flower abundance, and effective population size of 13 populations of Argentina anserina. The datasets include data from the field (pollinator visitation, flower abundance), histological data on pollen-pistil interactions from field-collected flowers, and population-level data on effective population size generated from ddRADseq analyses (see ). Data were collected across the span of three years (2019-2021).

### Excel Files:

**Name: pollinator_data_inputR2.xlsx**
Des...

全球范围内，花粉接收不足会限制显花植物的繁殖。花粉限制（pollen limitation, PL）的生态成因，例如传粉者稀缺、植物种群密度偏低，长期以来都是研究的核心议题。植物种群的遗传多样性可与生态因子协同作用，共同影响花粉限制的数量与质量组分，但相关实证研究案例仍较为匮乏。本研究针对一种常见草本的13个种群，评估了生态因子（花部丰度、传粉者访花频率）与遗传有效种群大小（genetic effective population size, N_E）对花粉限制的联合效应。我们采用组织学分析方法，利用1137朵花的5504个花柱样本，分离得到花粉限制的数量与质量组分，并将其与植物繁殖输出建立关联。传粉者访花频率与N_E存在交互作用共同调控花粉限制，但N_E的直接影响更为显著。种群有效规模越小，其面临的数量型花粉限制程度越强；在无传粉者的环境中开展的控制授粉实验显示，花粉数量是种群水平的固有属性……

本研究数据集涵盖：自然植物种群野外采集的传粉者访花率与花密度数据、野外采集花朵的花粉-花柱互作组织学数据、源自限制性位点相关DNA测序（restriction-site associated DNA sequencing, RAD-Seq）数据的种群基因组学指标（已既往发表），以及温室条件下开展的杂交实验数据。本研究使用R v.4.2.1完成数据分析，本数据集对应研究主题为：有效种群大小调控传粉服务对花粉限制的影响。

数据集还包含自然种群的传粉者访花数据、野外采集花朵的花粉-花柱互作组织学数据，以及种群属性数据（有效种群大小、花部丰度、海拔等）。同时附带了论文中分析所用的R代码。

## 数据与文件结构说明
本研究将花粉限制与13个鹅绒委陵菜（Argentina anserina）种群的传粉者访花频率、花部丰度及有效种群大小相关联。数据集涵盖：野外采集数据（传粉者访花率、花部丰度）、野外采集花朵的花粉-花柱互作组织学数据，以及通过双酶切限制性位点相关DNA测序（double digest RADseq, ddRADseq）分析得到的种群水平有效种群大小数据（详见原文标注缺失处）。数据采集周期为2019年至2021年，共计三年。

### Excel文件：
**文件名：pollinator_data_inputR2.xlsx**
描述……