MOLECULE， CELL， PLANT， SEEDS， RECOMBINANT POLYPEPTIDES， A METHOD FOR PRODUCING A POLYPEPTIDE， PLANT， METHOD FOR CONTROLLING WEEDS， THE USE OF NUCLEIC ACID PRODUCT OF THE UTILITY

In the present invention， HPPD polypeptides and plants containing them showing a full tolerance against one or more HPPD inhibitor herbicides belonging to various chemical classes are described. A set of mutant HPPD polypeptides have been designed which have either no or only a significantly reduced affinity to HPPD inhibitor herbicides and， at the same time， the rate of dissociation of the HPPD inhibitors of the mutant HPPD polypeptide is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow， tight-binding inhibitors but， instead of this， have become fully reversible inhibitors. In particular， isolated polynucleotides encoding mutant HPPD polypeptides conferring tolerance to HPPD inhibitor herbicides belonging to various chemical classes are provided. Additionally， amino acid sequences corresponding to the polynucleotides are encompassed. In the present invention， HPPD polypeptides and plants containing them showing a full tolerance against one or more HPPD inhibitor herbicides belonging to various chemical classes are described. A set of mutant HPPD polypeptides have been designed which have either no or only a significantly reduced affinity to HPPD inhibitor herbicides and， at the same time， the rate of dissociation of the HPPD inhibitors of the mutant HPPD polypeptide is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow， tight-binding inhibitors but， instead of this， have become fully reversible inhibitors. In particular， isolated polynucleotides encoding mutant HPPD polypeptides conferring tolerance to HPPD inhibitor herbicides belonging to various chemical classes are provided. Additionally， amino acid sequences corresponding to the polynucleotides are encompassed.

本发明公开了一类对羟基苯基丙酮酸双加氧酶（HPPD）多肽以及包含该类多肽的植物，这类植物对隶属于多种化学类别的一种或多种HPPD抑制剂类除草剂具有完全耐受性。研究人员设计了一组突变型HPPD多肽，这类多肽对HPPD抑制剂类除草剂要么完全无亲和力，要么仅具有显著降低的亲和力；与此同时，突变型HPPD多肽对HPPD抑制剂的解离速率提升至如此程度，以至于HPPD抑制剂不再发挥慢速结合型或慢速紧密结合型抑制剂的作用，反而转变为完全可逆的抑制剂。具体而言，本发明提供了编码突变型HPPD多肽的分离多核苷酸，这类多肽可赋予植物对隶属于多种化学类别的HPPD抑制剂类除草剂的耐受性。此外，本发明还涵盖了与该多核苷酸对应的氨基酸序列。本发明公开了一类对羟基苯基丙酮酸双加氧酶（HPPD）多肽以及包含该类多肽的植物，这类植物对隶属于多种化学类别的一种或多种HPPD抑制剂类除草剂具有完全耐受性。研究人员设计了一组突变型HPPD多肽，这类多肽对HPPD抑制剂类除草剂要么完全无亲和力，要么仅具有显著降低的亲和力；与此同时，突变型HPPD多肽对HPPD抑制剂的解离速率提升至如此程度，以至于HPPD抑制剂不再发挥慢速结合型或慢速紧密结合型抑制剂的作用，反而转变为完全可逆的抑制剂。具体而言，本发明提供了编码突变型HPPD多肽的分离多核苷酸，这类多肽可赋予植物对隶属于多种化学类别的HPPD抑制剂类除草剂的耐受性。此外，本发明还涵盖了与该多核苷酸对应的氨基酸序列。