BlurLab -- 3D Microscopy Simulation Package

BlurLab is an easy to use platform for generating simulated fluorescence microscopy data for use in mechanistic modeling visualization, image comparison, and hypothesis testing. The software accepts the 3D positions, intensities and labels of fluorescing objects that are produced by an underlying mechanistic model and transforms them into high quality simulated images. The program includes full 3D convolution with realistic (or even measured) point spread functions; inclusion of thermal, shot and custom noise spectra; simulations of mean and fully stochastic photobleacing; the ability to view scenes in wide-field and TIRF, and perform Z-slicing; and the ability to simulate FRAP experiments. The software provides a platform for adjusting and saving these simulated images, as well as a number of helpful, semi-automated features to make image simulation easy and less error prone. <br/><br/>This project includes the following software/data packages: <br/> <ul> <li> <a href="https://simtk.org/frs?group_id=659#pack_1069">BlurLab </a> </li> </ul>

BlurLab是一款易于使用的平台，旨在生成模拟荧光显微镜数据，以用于机制建模可视化、图像比较和假设检验。该软件能够接受由底层机制模型生成的荧光物体的三维位置、强度和标签，并将这些信息转化为高质量的模拟图像。该程序包括完整的3D卷积以及现实（甚至测量）的点扩散函数；包含热噪声、射击噪声和自定义噪声频谱；模拟平均和完全随机的光漂白；能够以宽场和共聚焦成像方式查看场景，并执行Z轴切片；以及模拟FRAP实验的能力。该软件提供了一个调整和保存这些模拟图像的平台，并提供了一系列有助于半自动化的功能，以简化图像模拟过程并降低出错率。本项目包括以下软件/数据包：

<ul>
<li><a href="https://simtk.org/frs?group_id=659#pack_1069">BlurLab</a></li>
</ul>