Engineering Patient-Specific Tissue Implants

We developed an approach to engineer completely personalized tissue implants that are based merely on the patient's own materials and cells. Here, an omentum is extracted from the patient in a safe, easy and quick procedure. The cells and the ECM materials of the tissue are separated and while the cells are reprogrammed to become pluripotent, the ECM is processed to become a personalized thermoresponsive hydrogel. The undifferentiated cells are then encapsulated within the hydrogel and efficiently differentiated to cardiomyocytes, neurons, and endothelial cells. Overall design: Examination of differentiation of iPSCs iside omentum hydrogel and matrigel