Knockout of the B1 subunit of the GA-binding protein (GABP) [ChIP-seq]

We knocked out different exons corresponding to different sections of GABP, a transcription factor known to bind to the telomerase reverse transcriptase (TERT) promoter with the G228 mutation. We investigated the effects of the knockouts in the regulation of TERT expression and other subunits of GABP. GABP genomic binding sites were determined through chromatin immunoprecipitation sequencing (ChIP-seq), and gene expression was determined through total RNA sequencing (RNA-seq). Combining data pertaining to GABP binding sites and gene expression provided insight into the molecular mechanisms of maintaining the length of telomeres in cancers. Overall design: ChIP-seq for 3 conditions. One condition was a knockout of the exon 2 of the B1 subunit of GABP followed by knock-in of TERT. One condition was a control sample that knocked out ROSA26. One condition was a control sample with no knockouts.