Mycorrhizal root enzyme activity, fungal community, and soil chemistry in a long-term warming and fertilization experiment in Arctic tundra at Toolik Lake, Alaska (July 2017)

Arctic nutrient cycling is heavily influenced by mycorrhizal processes. With warming, deciduous ectomycorrhizal (EcM) shrubs are expanding across the tundra, potentially increasing the prevalence of ectomycorrhizae while decreasing other dominant mycorrhizal types. Because carbon (C) and nutrient cycling in Arctic tundra are tightly coupled, changes in nutrient cycling induced by these shifts in mycorrhizal regime and function could impact the large stocks of soil carbon. This project characterized the responses of EcM nutrient cycling to long-term warming and nutrient addition. All data was collected from the Arctic Long Term Ecological Research Network (LTER) warming and fertilization experiment established in 1989. We paired measurements of EcM root function with EcM fungal identity. This dataset consists of potential activities of 5 enzymes (leucine aminopeptidase, N-acetyl glucosaminidase, acid phosphatase, laccase, and peroxidase) measured on Betula nana root tips colonized by EcM; EcM root tip abundance and fungal identity; and soil chemistry. Fungal internal transcribed spacer region (ITS) sequences used for taxonomic identification are archived in GenBank under the accession numbers MT278352-MT278808.

北极养分循环极大地受菌根过程影响。随着气候变暖，落叶外生菌根（ectomycorrhizal, EcM）灌木在苔原区域持续扩张，可能提升外生菌根的占比，同时抑制其他优势菌根类型。由于北极苔原的碳（carbon, C）循环与养分循环紧密耦合，菌根系统及其功能的上述变化所引发的养分循环改变，可能对土壤中巨量的碳库产生影响。本项目旨在阐明外生菌根养分循环对长期增温与养分添加的响应特征。所有数据均采集自1989年建立的、隶属于北极长期生态研究网络（Long Term Ecological Research Network, LTER）的增温与施肥实验样地。本研究将外生菌根根系功能的测定结果与外生菌根真菌的物种鉴定信息进行了配对整合。本数据集包含5种酶的潜在酶活数据：亮氨酸氨基肽酶、N-乙酰氨基葡萄糖苷酶、酸性磷酸酶、漆酶与过氧化物酶，这些酶活均在被外生菌根定殖的矮桦（Betula nana）根尖上测定；同时包含外生菌根根尖丰度、真菌物种鉴定信息，以及土壤化学性质数据。用于物种分类鉴定的真菌内转录间隔区（internal transcribed spacer region, ITS）序列已存档于GenBank，登录号为MT278352至MT278808。