Raw data for transcriptome analysis and molecular docking.

To assess the binding affinity of kaempferol with LuxS protein and blaCTX-M-27 protein, we conducted a molecular docking assay using Autodock4 software. The chemical structure of kaempferol was retrieved from the PubChem database, while the structures of E. coli LuxS protein and blaCTX-M-27 protein were obtained from UniProtKB. We assessed the binding capability between kaempferol and LuxS protein using Autodock Vina, Pymol was used to visualize the results.Transcriptome sequencing services were conducted by Novogene-Beijing platform. SY20 of ESBLs E.coli were treated 0.25MIC kaempferol and 0.25MIC kaempferol + 0.25MIC ceftiofur) procedure involved reactivating the test bacteria in 400mL of MH broth and incubating them at 37°C for 4 hours until reaching the early log phase. Subsequently, drug solutions were added to achieve the desired final concentration and then incubated at 37°C for an additional 4 hours. The cultures were then centrifuged at 4°C, the supernatant was discarded, and the samples were flash-frozen in liquid nitrogen. Utilizing established RNA extraction protocols. RNA integrity and total quantity were evaluated using an Agilent 2100 bioanalyzer. Ribosomal RNA (rRNA) was depleted from the total RNA to enrich for mRNA using probes, and libraries were constructed in a strand-specific manner. Subsequently, different libraries were pooled based on effective concentration and desired sequencing output for Illumina sequencing, which involved quality analysis of the sequencing, quantitative gene expression analysis, GO enrichment analysis, KEGG enrichment analysis, etc.