RNAseq measurement of cortisol induced gene expression change under knockout of glucocorticoid receptor (nr3c1). RNAseq measurement of cortisol induced gene expression change under knockout of glucocorticoid receptor (nr3c1)

We found that GR mediates most of the effects of the treatment, and paradoxically, that the transcriptome of cortisol-treated larvae is more like that of larvae lacking a GR than that of larvae with a GR, suggesting that the cortisol-treated larvae develop GR resistance. Overall design: To assess the role of the Glucocorticoid Receptor (GR) in such programming, we used CRISPR-Cas9 to produce a new frameshift mutation, GR369-.