RNA-seq analysis of Arabidopsis root with MBZ treatment

we reported the identification of a small molecule, Mebendazole (MBZ) that modulates GSA in Arabidopsis thaliana roots. To identify pathways immediately response to MBZ, we performed an mRNA-seq using roots of 14-days-old Arabidopsis grown on DMSO plates treated with DMSO or 10 µM MBZ for 4 hours. Differenetial expressed genes between DMSO and MBZ_4h treatments were analyzed. It has 8 samples in total, with 4 replicates for each treatment. 14 d old seedlings of Col-0 on DMSO plates were transferred to DMSO plates with 10 µM mebendazole or DMSO. After treatment for 4 h, root tissues were collected for RNA extraction and sequencing. Every treatment have 4 replicates.