Additional file 2 of The landscape of PBMC methylome in canine mammary tumors reveals the epigenetic regulation of immune marker genes and its potential application in predicting tumor malignancy

Additional file 2: Table S1. The information about dog donors providing blood samples used for MBD-seq. Table S2. Sequencing Coverage and Quality Statistics. Table S3. The statistics and genomic features of NT_DMR (2840 DMRs). Table S4. The statistics and genomic features of NB_DMR (3373 DMRs).Table S5. The statistics and genomic features of NC_DMR (1876 DMRs). Table S6. The statistics and genomic features of BC_DMR (168 DMRs). Table S7. The list of enriched immune-related terms with adj.p <0.1. Table S8. The list of hypermethylated DMRs in immune cell type markers (Panglao DB). Table S9. The list of immune-regulating DMGs inversely correlated with gene expression. Table S10. The list of primers designed for targeted BS-sequencing. Table S11. The information of unknown dog PBMC donors (used for validation sets of NT classifier). Table S12. The list of 127 DMRs which have high feature importance in BC classifier.

附加文件2：表S1 用于甲基化DNA结合蛋白测序（MBD-seq）的血液样本供体犬信息。表S2 测序覆盖度与质量统计。表S3 NT_DMR（共2840个差异甲基化区域（Differentially Methylated Region, DMR））的统计信息及基因组特征。表S4 NB_DMR（共3373个差异甲基化区域）的统计信息及基因组特征。表S5 NC_DMR（共1876个差异甲基化区域）的统计信息及基因组特征。表S6 BC_DMR（共168个差异甲基化区域）的统计信息及基因组特征。表S7 校正后P值（adj.p）小于0.1的富集免疫相关术语列表。表S8 免疫细胞类型标记基因中高甲基化差异甲基化区域列表（来自Panglao数据库（Panglao DB））。表S9 与基因表达呈负相关的免疫调控差异甲基化基因（Differentially Methylated Gene, DMG）列表。表S10 靶向亚硫酸氢盐测序（Bisulfite Sequencing）引物设计列表。表S11 未知供体犬外周血单个核细胞（Peripheral Blood Mononuclear Cell, PBMC）供体信息（用于NT分类器的验证集）。表S12 在BC分类器中具有高特征重要性的127个差异甲基化区域列表。