Complete genome sequence of highly virulent phage against Staphylococcus aureus MRSA isolated from Egypt

One of the most resistant bacteria to antibiotics is Methicillin-Resistant Staphylococcus aureus (MRSA). Staphylococcus aureus is an important prevalent pathogen that can cause a variety of infectious diseases in both humans and animals. The aim of the study was to isolate and characterize a bacteriophage as an alternative antibacterial agent against S.aureus MRSA. Different types of samples were collected from different clinical sources such as skin, ear wound, blood and urine were screened for Staphylococcus aureus MRSA. Isolates of S. aureus MRSA were obtained and defined by vitek 2 and to 16s rRNA sequencing. Bacteriophage was isolated from waste water samples that had been collected from many places and governorates in Egypt. S. aureus ATCC OQ546500 (MRSA), was used to test the infectivity of the isolated phage. A lytic phage, designated VB_SauP_ASUmrsa123, was isolated from waste water from a canal in Bahtim, Qalyubia, Egypt. The morphology of the isolated phage vB_SauP_ASUmrsa123 was determined by Transmission electron microscopy revealed that the phage has short tail belongs to the family Podoviridae. In this study, we report the whole genome sequencing and annotation of phage VB_SauS_ vB_SauP_ASUmrsa123