Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [scRNA-seq]

Purpose: The goal of this study is to determine alterations in heart endothelial cell transcripts and cell populations associated with aging. Methods: Endothelial single cell mRNA profiles of 3 month and 24 month old wild-type mice were generated by 10X Genomics Results: Using an optimized data analysis workflow, we mapped about 50 thousand reads per cell Conclusions: Our study represents the first analysis of aged cardiac endothelial cell transcriptomes using single cell RNA-seq technology, identifiying specific transcripts and cell populations affected by age. Endothelial cell mRNA profiles of 3 month old and 24 month old mice研究目的：本研究旨在明确与衰老相关的心脏内皮细胞转录本及细胞群体的表达变化。 实验方法：通过10X Genomics技术，获取3月龄与24月龄野生型小鼠的内皮细胞单细胞信使RNA（mRNA）表达谱。 实验结果：采用优化后的数据分析流程，我们对每个细胞约5万条测序读段（reads）完成了比对分析。 研究结论：本研究为首例利用单细胞RNA测序（single cell RNA-seq）技术开展的老年心脏内皮细胞转录组分析，成功鉴定出受衰老影响的特异性转录本与细胞群体。 本数据集涵盖3月龄与24月龄小鼠的内皮细胞mRNA表达谱。