Table 1 in German CULex pipienS biotype MoLeStUS and CULex torrentiUM are vector-competent for Usutu virus

Table 1 Infection, dissemination, and transmission rates of mosquitoes infected with the German USUV Africa 2 strain
Blood meal virus titer (TCID 50 /ml)Mosquito speciesDpiInfection rate (%) (95% CI)Mean viral load bodies (viral copies/µl of total RNA)Dissemination rate (%) (95% CI)Mean viral load legs plus wings (viral copies/µl of total RNA)Transmission rate (%) (95% CI)High titer 10 7.4Culex pipiens biotype molestus a148/10 (80.0) (44.4–97.5)6.9 × 10 53/8 (37.5) (8.5–75.5)9.0 × 10 33/3 (100) (29.2–100)214/6 (66.7) (22.3–95.7)5.6 × 10 54/4 (100) (39.7–100)1.5 × 10 43/4 (75.0) (19.4–99.4)Cx.pipiens biotype molestus b1613/16 (81.3) (54.4–96.0)1.9 × 10 613/13 (100) (75.3–100)7.8 × 10 42/13 (15.4) (1.9–45.4)218/10 (80.0) (44.4–97.5)8.1 × 10 58/8 (100) (63.1–100)7.8 × 10 44/8 (50.0) (15.7–84.3)Aedes aegypti d140/53 (0) (0–6.7)NANANANA214/22 (18.2) (5.2–40.3)2.3 × 10 51/4 (25.0) (0.6–80.6)5.5 × 10 30/1 (0) (0–97.5)Low titer 10 5.1Cx.pipiens biotype molestus a142/36 (5.6) (0.7–18.7)1.2 × 10 20/2 (0) (0–84.2)NANA211/19 (5.3) (0.7–18.7)5.4 × 10 10/1 (0) (0–84.2)NANACx.torrentium c141/8 (12.5) (0.3–52.7)2.8 × 10 10/1 (0) (0–97.5)NANA211/8 (12.5) (0.3–52.7)3.9 × 10 61/1 (100) (2.5–100)4.7 × 10 41/1 (100) (2.5–100)
Transmission rates include results from the saliva inoculation on Vero cells and from the RT-qPCRs of cell culture supernatants.All mosquitoes were incubated for 14/16 or 21 days.Absolute quantification of virus copies/µl of total RNA was performed via an RT-qPCR-based calibration curve
CI confidence interval, dpi days post infection, NA not applicable
a Cx.pipiens biotype molestus laboratory colony from“Wendland,” Lower Saxony,Germany
b Cx.pipiens biotype molestus laboratory colony from Novi Sad,the Republic of Serbia
c Cx.torrentium field-collected colony near Berlin and Bonn,North Rhine-Westphalia,Germany
d Ae. aegypti laboratory colony from Malaysia (Bayer CropScience,Langenfeld,Germany)