Root associated fungi in Arctic Glacier Forlands raw sequences
收藏Mendeley Data2024-04-13 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.7sqv9s4qw
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DNA was extracted from the entire plant root system using a modified CTAB extraction protocol , and further purified using the E.Z.N.A. Soil DNA kit (Omega Bio-tek, USA) following the manufacturer's protocol. A negative control was used during extraction procedure and included in PCR and sequencing. We amplified the internal transcribed space 2 (ITS2) region of the nuclear ribosomal rDNA using primers fITS7a and ITS4. PCR procedures, library preparation and Multiplex Identification DNA-tags. Paired-end sequencing (2 × 300) was performed on an Illumina MiSeq sequencer
创建时间：
2023-06-28
