Table_2_Screening Oat Genotypes for Tolerance to Salinity and Alkalinity.DOC

A set of four experiments was conducted to develop methods for screening oat tolerance to salt and alkali and the following results were obtained. (1) In experiment 1, 68.5 mmol L-1 salt and 22.5 mmol L-1 alkali were identified as appropriate concentrations for determining oat tolerance to salinity and alkalinity during germination. (2) These concentrations were used in experiment 2 to screen 248 oat genotypes and 21 were identified to be tolerant to salinity and alkalinity in germination. (3) In experiment 3, one salt treatment, 40 L of Na2SO4:NaCl (1:1), 150 mmol L-1, was found to be optimal for screening oat tolerance to salinity during growth and development. For alkalinity tolerance, the optimal treatment was 40 L of Na2CO3:NaHCO3 (1:1) at 75 mmol L-1. (4) No significant correlation was found between tolerances at the germination and adult stages or between tolerances to salt and alkali. Three lines were found to be tolerant to both salt and alkali in both germination and adult stages. (5) In experiment 4, 25 out of 262 oat genotypes were found to be tolerant to both salinity and alkalinity. (6) GGE biplot analysis was found to be effective in interpreting the multivariate data and the plastic cone-container system was found to be cost-effective system for screening adult plant tolerance to salt and alkali. (7) The symptoms of salt stress and alkali stress were found to be different; alkali stress mainly reduces the chlorophyll content, while salinity mainly disrupts water absorption.

本系列实验旨在开发筛选燕麦对盐碱耐受性的方法，并取得了以下成果：（1）在实验1中，确定了68.5 mmol·L^-1的盐浓度和22.5 mmol·L^-1的碱浓度，作为评估燕麦在萌发阶段对盐碱耐受性的适宜浓度。（2）在实验2中，采用上述浓度对248个燕麦基因型进行了筛选，其中21个基因型表现出在萌发阶段对盐碱的耐受性。（3）实验3中，发现40 L的Na2SO4:NaCl（1:1）溶液，浓度为150 mmol·L^-1，是筛选燕麦在生长发育阶段对盐碱耐受性的最佳处理方法；对于碱性耐受性，最佳处理方法为40 L的Na2CO3:NaHCO3（1:1）溶液，浓度为75 mmol·L^-1。（4）未发现萌发阶段与成熟阶段耐受性之间存在显著相关性，亦未发现盐碱耐受性之间存在显著相关性。有三种燕麦基因型在萌发和成熟阶段均表现出对盐碱的耐受性。（5）在实验4中，262个燕麦基因型中有25个表现出对盐碱的双重耐受性。（6）GGE 双因素图分析被证明是解析多变量数据的有效工具，而塑性圆锥容器系统则被证明是一种成本效益较高的筛选成年植物对盐碱耐受性的系统。（7）盐碱胁迫的症状存在差异；碱性胁迫主要降低叶绿素含量，而盐碱胁迫主要干扰水分吸收。