Identification of a UDP-glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat

Supporting information/data for the publication: Identification of a UDP-glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat Table S1 Analysis of variance for the evaluated traits.Table S2 Pairwise correlations of the evaluated traits.Table S3 Best linear unbiased estimates of the evaluated traits in 2019 and 2020.Table S4 Best linear unbiased estimates of the evaluated traits across years.Table S5 Deoxynivalenol (DON) and DON-3-O-glucoside (D3G) contents and the ratio of D3G/DON.Table S6 The mapped region significantly associated with Fusariumhead blight resistance on chromosome 7D.Table S7 The mapped region significantly associated with deoxynivalenol resistance on chromosome 5D.Table S8 Allelic diversity of the uridine diphosphate (UDP)-glucosyltransferase (UGT) for the 305 Ae. tauschii accessions and 18 hexaploid wheat lines.Table S9 List of phenotyped Ae. tauschii accessions. Figure S1 Pearson’s correlation coefficients of the Aegilops tauschii accessions for Fusarium head blight and deoxynivalenol severities and other traits.Figure S2 k-mer-based association genetics for Fusarium head blight severity.Figure S3 Amino acid alignment of the allelic diversity at the UGT for the sequenced Ae. tauschii accessions and wheat cv. Chinese Spring.Figure S4 Amino acid alignment of the chromosome ‘5D UGTs’ and the homoeologous proteins at chromosomes 5A and 5B of Chinese Spring.Figure S5 Sequence of the codon-optimized Ae. tauschii UGT AET5Gv20385300, the deduced protein sequence and primers used for in vitro mutagenesis.Figure S6 Functional evaluation of the homoeologous uridine diphosphate (UDP)-glucosyltransferases (UGT) at chromosomes 5A (TraesCS5A01G149600), 5B (TraesCS5B01G148300) and 5D (TraesCS5D01G143300, AET5Gv20385300).Figure S7 Sequences of the codon-optimized UGTs TraesCS5A01G149600 and TraesCS5B01G148300.