Robust RNA editing via recruitment of endogenous ADARs using circular guide RNAs

We engineered circular ADAR recruiting guide RNAs (cadRNAs) that efficiently recruit endogenous ADARs to edit specific sites on target RNA Overall design: We tested two Circular ADAR recruiting guide RNAs (cadRNAs). "Circular 100,50" refers to a cadRNA targeting the RAB7A transcript with an antisense domain of length 100 bp and containing a centrally located C-mismatch (position 50). Similarly, "Circular 200,100" refers to a cadRNA targeting the RAB7A transcript with an antisense domain of length 200 bp and containing a centrally located C-mismatch (position 100). "293FT" refers to the control sample, i.e., HEK-293FT cells that were not treated with any cadRNA.