Whole embryo depletion of Taf15 in gastrula and neurula stage Xenopus tropicalis embryos

We examined the role of Taf15 in in gastrula and neurula stage Xenopus tropicalis embryos. Overall design: To determine the role of Taf15 in early Xenopus development, we used RNA-sequencing (RNA-seq) from single embryos depleted of M- and Z-Taf15, using reagents that targeted either all mRNA by inhibiting translation with Morpholino antisense and mismatch oligonucleotides (MOs) or only zygotic function using splice-blocking MOs or CRISPR-mediated mutagenesis.