Skeletal muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro

Raw data for the article published in Nature Biomedical Engineering. Title: Skeletal muscle regeneration via the chemical induction and expansion of myogenic stem cells<br>in situ or in vitro.<br>AbstractMuscle loss and impairment resulting from traumatic injury can be alleviated by therapies using muscle stem cells. However, collecting sufficient numbers of autologous myogenic stem cells and expanding them efficiently has been challenging. Here we show that myogenic stem cells (predominantly Pax7+ cells)—which were selectively expanded from readily obtainable dermal fibroblasts or skeletal muscle stem cells using a specific cocktail of small molecules and transplanted into muscle injuries in adult, aged or dystrophic mice-led to functional muscle regeneration in the three animal models. We also show that sustained release of the small-molecule cocktail in situ through polymer nanoparticles led to muscle repair by inducing robust activation and expansion of resident satellite cells. Chemically induced stem cell expansion in vitro and in situ may prove to be advantageous for stem cell therapies that aim to regenerate skeletal muscle and other tissues. <br><br>

本数据集为发表于《自然·生物医学工程》（Nature Biomedical Engineering）的论文配套原始数据，论文标题为《通过化学诱导与扩增肌源性干细胞（myogenic stem cells）实现原位或体外骨骼肌再生》。
摘要：创伤性损伤引发的肌肉缺损与功能障碍，可通过肌肉干细胞疗法得到缓解。然而，获取足量自体肌源性干细胞并实现其高效扩增始终是领域内的技术挑战。本研究表明，借助特定小分子组合，可从易获取的皮肤成纤维细胞（dermal fibroblasts）或骨骼肌干细胞中选择性扩增肌源性干细胞（主要为Pax7阳性细胞（Pax7+ cells））；将此类细胞移植至成年、老年或肌营养不良小鼠的肌肉损伤部位后，可在三种动物模型中实现功能性肌肉再生。本研究同时证实，通过聚合物纳米颗粒实现小分子组合的原位缓释，可通过强力激活并扩增驻留卫星细胞，进而实现肌肉修复。体外与原位的化学诱导干细胞扩增策略，有望为旨在再生骨骼肌及其他组织的干细胞疗法带来显著优势。