Alingment of COI sequences of Ch. plumosus group species and Tables 1-5.

The alignment that was used for analysis for paper "Karyotype characteristics and gene COI sequences of <i>Chironomus bonus </i>Shilova &amp; Dzhvarsheishvili, 1974<i> </i>(Diptera, Chironomidae) from the South Caucasus". The alignment consists of DNA data (COI gene sequences) from both GenBank and BOLD (Ratnasingham and Hebert 2007) databases for the species <i>Chironomus dorsalis</i> Meigen, 1818 (JN887047.1); <i>Ch. balatonicus</i> (JN016826.1); <i>Ch. muratensis</i> (AF192194.1); <i>Chironomus </i>sp. prope <i>agilis </i> (AF192190.1); <i>Ch. borokensis</i> (AB740261.1);<i> Ch. usenicus</i> (JN016807.1, JN016809.1, JN016808.1); <i>Ch. entis</i> (KF278213.1, KF278212.1, KJ085531.1, KJ087284.1, KJ089893.1, GBDPC429-14, MGOCF102-16); <i>Ch. plumosus</i> (AB740263.1, AB740262.1, JN016830.1, JN016829.1, CHBAL014-20, CHIFI298-16, CHIFI299-16, LEFIJ3947-16, LEFIJ3948-16, PGBAL006-19, PGBAL007-19, PGBAL009-19, PGCBG089-20, BSCHI661-17, BSCHI063-11, BSCHI115-17, BSCHI219-17, BSCHI284-17, BSCHI350-17, BSCHI517-17, BSCHI644-17, GBDP44143-19, GBDP44180-19, LC050899.1, LC050900.1, JCDB364-15, JCDB363-15, GBDP11685-12, GBDP11686-12, GBDP11687-12, GBDP12282-12, XJDQD1039-18, XJDQD1037-18, XJDQD1038-18, XJDQD1036-18, MN750315.1, GBDPC430-14, SDP408034-15, GBDPC133-14, GBDPC138-14, GBDPC144-14, GBDPC166-14); and <i>Pagastiella orophila </i>(Edwards, 1929)<i> </i>(JN265047.1).<br>Table 1. Estimates of evolutionary divergence between sequences of true <i>Ch. plumosus </i>cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 2. Estimates of evolutionary divergence between sequences of <i>Ch. entis </i>cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 3. Estimates of evolutionary divergence between sequences of <i>Ch. borokensis </i>cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 4. Estimates of evolutionary divergence between sequences of <i>Ch. plumosus </i>from Finland and sequences of <i>Ch. balatonicus</i>, <i>Ch. muratensis</i> and <i>Ch. agilis</i> 2. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980).<br>Table 5. Estimations of the age of the most recent common ancestors (TMRCAs) for DNA clades.

本序列比对用于论文《南高加索地区<i>Chironomus bonus</i> Shilova & Dzhvarsheishvili, 1974（双翅目：摇蚊科）的核型特征与COI基因序列》的分析工作。该比对包含采自GenBank（基因库）与BOLD（生命条形码数据系统，Ratnasingham与Hebert, 2007）数据库的以下物种的DNA序列数据（COI基因序列）：<i>Chironomus dorsalis</i> Meigen, 1818（登录号JN887047.1）；<i>Ch. balatonicus</i>（登录号JN016826.1）；<i>Ch. muratensis</i>（登录号AF192194.1）；<i>Chironomus sp. prope agilis</i>（登录号AF192190.1）；<i>Ch. borokensis</i>（登录号AB740261.1）；<i>Ch. usenicus</i>（登录号JN016807.1、JN016809.1、JN016808.1）；<i>Ch. entis</i>（登录号KF278213.1、KF278212.1、KJ085531.1、KJ087284.1、KJ089893.1、GBDPC429-14、MGOCF102-16）；<i>Ch. plumosus</i>（登录号AB740263.1、AB740262.1、JN016830.1、JN016829.1、CHBAL014-20、CHIFI298-16、CHIFI299-16、LEFIJ3947-16、LEFIJ3948-16、PGBAL006-19、PGBAL007-19、PGBAL009-19、PGCBG089-20、BSCHI661-17、BSCHI063-11、BSCHI115-17、BSCHI219-17、BSCHI284-17、BSCHI350-17、BSCHI517-17、BSCHI644-17、GBDP44143-19、GBDP44180-19、LC050899.1、LC050900.1、JCDB364-15、JCDB363-15、GBDP11685-12、GBDP11686-12、GBDP11687-12、GBDP12282-12、XJDQD1039-18、XJDQD1037-18、XJDQD1038-18、XJDQD1036-18、MN750315.1、GBDPC430-14、SDP408034-15、GBDPC133-14、GBDPC138-14、GBDPC144-14、GBDPC166-14）；以及<i>Pagastiella orophila</i> (Edwards, 1929)（登录号JN265047.1）。
表1 真实<i>Ch. plumosus</i>分支序列间的进化分歧估计值。表格展示了序列间每一位点的碱基替换百分率（%）。分析采用Kimura双参数模型（Kimura, 1980）完成。
表2 <i>Ch. entis</i>分支序列间的进化分歧估计值。表格展示了序列间每一位点的碱基替换百分率（%）。分析采用Kimura双参数模型（Kimura, 1980）完成。
表3 <i>Ch. borokensis</i>分支序列间的进化分歧估计值。表格展示了序列间每一位点的碱基替换百分率（%）。分析采用Kimura双参数模型（Kimura, 1980）完成。
表4 芬兰<i>Ch. plumosus</i>序列与<i>Ch. balatonicus</i>、<i>Ch. muratensis</i>及<i>Ch. agilis</i> 2序列间的进化分歧估计值。表格展示了序列间每一位点的碱基替换百分率（%）。分析采用Kimura双参数模型（Kimura, 1980）完成。
表5 DNA进化支的最近共同祖先年代（Time to the Most Recent Common Ancestor, TMRCAs）估计值。