Table_2_High levels of Bifidobacteriaceae are associated with the pathogenesis of Parkinson’s disease.docx

BackgroundThe diagnosis of Parkinson’s disease (PD) is complex and there are no biomarkers for early identification. Many studies have reported altered gut microbiota in patients with PD compared with healthy individuals. However, results from previous studies vary across countries.AimsThe aim of this study was to identify gut microbiota biomarkers that could be used as a marker for the diagnosis of PD.MethodsFirstly, the differential gut microbiota was obtained by meta-analysis, and then the results of meta-analysis were validated through metagenomic cohort. Finally, the ROC curve was drawn based on the metagenomic validation results.ResultsThe meta-analysis showed a lower relative abundance of Prevotellaceae (p < 0.00001) and Lachnospiraceae (p = 0.002), and a higher of Ruminococcaceae (p < 0.00001), Christensenellaceae (p = 0.03), Bifidobacteriaceae (p < 0.00001), and Verrucomicrobiaceae (p = 0.02) in patients with PD. Only Bifidobacteriaceae was also at high levels in the validation cohort of the metagenome. Meanwhile, three species from the Bifidobacteriaceae, including Scardovia_inopinata (p = 0.022), Bifidobacterium_dentium (p = 0.005), and Scardovia_wiggsiae (p = 0.024) were also high. The ROC curve showed that the three species (71.2%) from Bifidobacteriaceae had good predictive efficiency for PD.ConclusionElevated Bifidobacteriaceae may be associated with PD. Elevated three species from the Bifidobacteriaceae, including Scardovia_inopinata, Bifidobacterium_dentium and Scardovia_wiggsiae may provide new potential biomarkers for the diagnosis of PD.

背景：帕金森病（PD）的诊断复杂，目前尚无早期识别的生物标志物。众多研究报道，与健康个体相比，帕金森病患者存在肠道菌群失调现象。然而，先前研究的结果在不同国家间存在差异。目标：本研究旨在识别可用于帕金森病诊断的肠道菌群生物标志物。方法：首先，通过荟萃分析获得差异性的肠道菌群，然后通过宏基因组队列验证荟萃分析的结果。最后，基于宏基因组验证结果绘制ROC曲线。结果：荟萃分析显示，帕金森病患者Prevotellaceae（p < 0.00001）和Lachnospiraceae（p = 0.002）的相对丰度降低，而Ruminococcaceae（p < 0.00001）、Christensenellaceae（p = 0.03）、Bifidobacteriaceae（p < 0.00001）和Verrucomicrobiaceae（p = 0.02）的相对丰度升高。在宏基因组验证队列中，仅Bifidobacteriaceae保持高水平。同时，来自Bifidobacteriaceae的三个物种，包括Scardovia_inopinata（p = 0.022）、Bifidobacterium_dentium（p = 0.005）和Scardovia_wiggsiae（p = 0.024）也表现出高水平。ROC曲线显示，来自Bifidobacteriaceae的这三个物种（71.2%）对PD具有良好的预测效率。结论：Bifidobacteriaceae水平的升高可能与PD相关。Bifidobacteriaceae中的三个物种，包括Scardovia_inopinata、Bifidobacterium_dentium和Scardovia_wiggsiae的升高可能为PD的诊断提供新的潜在生物标志物。