Gene expression profiles of WT and CREPT deleted Lgr5+ intestinal stem cells

Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPT in ISCs, we isolated WT and CREPT deleted Lgr5+ ISCs (Lgr5-CREPTKO) to perform Next generation sequencing. Overall design: To verify the role of CREPT in Lgr5+ ISCs, we knock out CREPT in Lgr5-GFP+ cells by intraperitoneal injection of Tamoxifen into mice, and sorted the Lgr5-GFPhigh cells from disaggregated WT and Lgr5-CREPTKO intestines. Total RNAs were extracted from sorted cells, and sequenced by BGISEQ-500.