Microsatellite genotypes and partial COI fragments for 5 cod species in the Chukchi Sea, Arctic Integrated Ecosystem Research Program, 2019

These data are part of the Arctic IES component of the Arctic Integrated Ecosystem Research Program (IERP), sponsored by the North Pacific Research Board (NPRB), Collaborative Alaskan Arctic Studies Program (formerly the North Slope Borough/Shell Baseline Studies Program), Bureau of Ocean Energy Management (BOEM), and the Office of Naval Research Marine Mammals and Biology Program. Initially, Arctic Cod (Boreogadus saida) was collected from the Chukchi Sea for genetic population structure analyses. Bulk samples of approximately 50 fish were retained from each midwater trawls. Cod samples were collected in August and September, 2019 for species identification. These samples were examined at 15 microsatellite loci and genotypes were recorded in an excel spreadsheet (two fragment sizes for each specimen). Additionally, phase 2 of the project was initiatiated, because the microsatellite analysis detected other cod species in the Arctic Cod sample. Fin clips from other cod projects were collected for genetic analysis to verify species identification (n=1545). mtDNA was examined at a partial (100 base pair) COI fragment and aligned with known sequences from other possible cods. Sequences are stored in a text file.

本数据集隶属于由北太平洋研究委员会（North Pacific Research Board, NPRB）、阿拉斯加北极协作研究计划（前身为北坡自治市/壳牌基线研究计划）、海洋能源管理局（Bureau of Ocean Energy Management, BOEM）以及海军研究办公室海洋哺乳动物与生物学项目资助的北极综合生态系统研究计划（Arctic Integrated Ecosystem Research Program, IERP）下属的北极IES（Arctic IES）组分。
最初，研究团队从楚科奇海采集北极鳕（Arctic Cod, Boreogadus saida），用于种群遗传结构分析。每次中层拖网作业后均留存约50尾鱼类组成的混合样本。2019年8月至9月期间，研究人员采集鳕形目样本以开展物种鉴定工作。
研究人员对这批样本的15个微卫星位点（microsatellite loci）进行了基因分型，并将基因型数据记录于Excel电子表格中，每份标本对应两个片段长度。此外，由于微卫星分析在北极鳕样本中检测到了其他鳕形目物种，本项目第二阶段正式启动：研究人员收集了其他鳕形目研究项目的鳍组织样本，用于遗传分析以验证物种鉴定结果，样本量n=1545。
本研究对线粒体DNA（mtDNA）的一段100碱基对的COI片段进行了检测，并与其他潜在鳕形目物种的已知序列完成比对，所有测序序列均存储于文本文件中。