RNA deep sequencing data of the whole transcriptomes of the aa3 mutant, aa3 mprA double mutant, and aa3 rel double mutant strains of Mycobacterium smegmatis MC2 155

To identify the effect of additional deletion of rel and mprA in the aa3 mutant strain lacking the aa3 cytochrome c oxidase of the respiratory electron transport chain of Mycobacterium smegmatis MC2 155, we constructed the aa3 rel double mutant and aa3 mprA double mutant and profiled the transcriptomes of the aa3 mutant, aa3 mprA double mutant and aa3 rel double mutant strains. Our comparative RNA sequencing analysis revealed that expression of most ribosomal proteins was induced in the aa3 rel double mutant and aa3 mprA double mutant relative to the aa3 mutant. Overall design: Comparison of gene expression between the aa3 mutant, aa3 mprA double mutant, and aa3 rel double mutant strains of Mycobacterium smegmatis that are aerobically grown.