Replication Data for: Molecular characterization of rotavirus group A strains circulating prior to vaccine introduction in rural coastal Kenya, 2002-2013
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https://doi.org/10.7910/DVN/LVGYYW
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These data were derived from stool samples collected from children with acute gastroenteritis, who reported to Kilifi County Hospital, with diarrhoea symptoms. The surveillance was conducted between January 2002 to December 2004 and January 2010 to December 2013. The study was aimed at describing the distribution, circulation patterns and diversity of group A rotavirus (RVA) strains circulating in this rural set up. This will ultimately form the basis of estimating the performance of a Rotavirus vaccine that was introduced in mid-2014, in Kenya. Sequence data was derived from Rotavirus positive samples identified by enzyme immunoassay method (ELISA) and subsequently sequenced by the di-deoxy Sanger method. After read assembly and sequence clean-up, G (derived from the VP7 glycoprotein) and P (derived from VP4 protease sensitive protein), genotypes were determined by use of NCBI BLAST and RotaC v2 an established online RVA genotyping tool. The data was then analyzed in excel and use of other sequence analysis tools including MEGA v7 for construction of phylogenetic trees.
创建时间:
2018-11-13



