Effects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry

We combined tissue culture and flow cytometry to assess the activities of various temperatures, chemicals, and disinfectants on the viability and infectivity of spores of Encephalitozoon intestinalis. Surfanios and benzalkonium chloride, disinfectants currently used in the hospital, were remarkably efficient in destroying spore viability and infectivity.