Ing4-deficiency promotes a quiescent yet transcriptionally poised state in hematopoietic stem cells

Bone marrow from 8-10 mice was harvested and pooled, and lineage depleted. 1,000-10,000 LT- and ST-HSCs were sorted separately into Buffer RLT Plus (Qiagen) for both WT or Ing4-/- mice. Total RNA was isolated with the RNeasy Micro column (QIAGEN). Library construction, sequencing by Illumina HiSeq, and initial bioinformatics analyses were conducted by GENEWIZ. Counts obtained were used to perform Gene Set Enrichment Analysis. Ing4-/- LT- and ST-HSCs were compared together against WT LT- and ST-HSCs.