Influenced genes by miR663a

An increasing number of studies have demonstrated that miRNAs may act as oncogenes or anti-oncogenes in various types of cancer, including colon carcinoma (CC). However the clinical and biological significance of miR663a in the prognosis of CC and its underlying mechanisms remain unknown. In our study, it was found that miR663a was significantly downregulated in CC, especially in metastatic CC tissues. MiR663a overexpression not only inhibited the proliferation and migration/invasion of CC cells in vitro, but also inhibited tumor growth and metastasis of CC cells in vivo. However the mechanism under that was still unknown. So miR663a target genes were analyzed in our project. To explore the target genes of miR663a which mediated its function, we overexpressed miR663a and its inhibitor in HCT116 cells and did the cDNA array to screen out the genes whose expression level was inhibited by miR663a overexpression and promoted by miR663a inhibition.