Microglia and infiltrating monocytes gene expression profiling during EAE progress

The project was designed to figure out the role of microglia in brain inflammation (EAE model). The first step was to find genes/pathways that are upregulated in the microglia during EAE progress but absent in the infiltrating monocytes. These genes/pathways may provide clues for studying the unique function of microglia in the brain inflammation that is disctinct from the infiltrating monocytes. Two biological replicates were included for every sample. Microglia cells from the spinal cord of MOCK immunized mice are used as healthy controls. Microglia and infiltrating monocytes were collected at three time points during EAE progression: preclinical (EAE onset), acute (peak), chronic.