METTL3 mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling (Ribosome-Seq)

To investigate the effect of METTL3 function in the regulation of neutrophil activation, we established neutrophil METTL3 knock out mice by crossing METTL3flox/flox mice with Lyzm-cre mice. We then performed gene expression profiling analysis using data obtained from Ribosome-seq of isolated neutrophil from METTL3f/f and METTL3f/f Lyzm-Cre mice . Comparative gene expression profiling analysis of Ribosome-seq data and RNA-seq data for METTL3flox/flox neutrophil and METTL3flox/flox-Lyzmcre neutrophil.为探究METTL3功能在中性粒细胞活化调控中的作用，我们通过将METTL3flox/flox小鼠与Lyzm-cre小鼠杂交，构建了中性粒细胞特异性METTL3敲除小鼠。随后，我们利用分离自METTL3f/f与METTL3f/f Lyzm-Cre小鼠的中性粒细胞的核糖体测序（Ribosome-seq）数据开展基因表达谱分析。同时，我们针对METTL3flox/flox中性粒细胞与METTL3flox/flox-Lyzmcre中性粒细胞的核糖体测序数据及RNA测序（RNA-seq）数据，完成了比较基因表达谱分析。