Research Data: Thermophoretic Migration of Vesicles Depends on Mean Temperature and Head Group Chemistry

This file contains the datasets for the Nature Communications article “Thermophoretic Migration of Vesicles Depends on Mean Temperature and Head Group Chemistry” by Emma L. Talbot, Jurij Kotar, Lucia Parolini, Lorenzo Di Michele and Pietro Cicuta. DOI: 10.1038/NCOMMS15351. The dataset contains raw images relating to the motion of vesicles in thermal gradients. Each compressed folder contains full resolution .tiff images of the fluorescence microscopy data for these vesicles with reference to the figure that data was used in within the main manuscript or supporting information. Each pixel is equal to 0.145 micrometers. Data for fluorescence measurements or zeta-potential measurements (Fig. 5 and Supplementary Fig. 9) are given in an excel spreadsheet. Please refer to the paper for further details. Images are labelled as follows for figures in the main manuscript: Fig 1c – “State_z_zh=X” (e.g. Initial_z_zh=0.05) where “State” can be either initial or steady state and refers to whether the data was recorded at time t=0 min or after steady state was reached, “X” refers to the height fraction z/zh above the cold plate i.e. z_zh=0.00 would be the cold plate. Fig 1d and Supplementary Fig 8– “DiPhyPC_TcX_ThY_dT=Z“ (e.g. DiPhyPC_Tc15_Th45_dT=3.0K) where DiPhyPC is the lipid type, “X” is the temperature of the cold plate in ˚C, “Y” is the temperature of the hot plate in ˚C, and “Z” is the temperature difference from that of the cold plate. Fig 3 - “Lipid_Tbar_X_runY_sliceZ” (e.g. DGDG_Tbar_10_run1_slice1) where “Lipid” is the lipid type (DGDG, SQDG, DiPhyPC, DPPC, DMPC, DOPC, DOPG, or POPG), “X” is the mean temperature between the hot and cold plates e.g. 10 refers to 10˚C, “Y” is the run number (between 1 and 5), “Z” is the slice number where slice 1 is at the cold plate and slice 19 at the hot plate. Fig 4– Folders contain data for “Lipid_T=XC_frameY” (e.g. DMPC_T=6C_frame1), where “Lipid” is the lipid type or mixture, “X” is the temperature of the sample and “Y” is the frame number. Frames were acquired at 10fps. Fig 6 and Supplementary Fig 7 – “Lipid_TcX_ThY_t=Zmin_sliceA” (e.g. DOPC_Tc25_Th55_t=0min_slice1) where “Lipid” is the lipid type, “X” is the temperature of the cold plate in ˚C, “Y” is the temperature of the hot plate in ˚C, “Z” is the time in minutes and “A” is the slice number where slice 1 is at the cold plate and slice 19 at the hot plate. Fig 7 – “Lipid1_Lipid2_Tbar_X_runY_sliceZ” (e.g. DOPC_DOPG_Tbar_15_run1_slice1) where “Lipid1” is the first lipid type and “Lipid2” the second lipid type in vesicles of lipid 1/lipid 2/chol. “X” is the mean temperature between the hot and cold plates e.g. 10 refers to 10˚C, “Y” is the run number (between 1 and 5), “Z” is the slice number where slice 1 is at the cold plate and slice 19 at the hot plate. Fig 8 – Folders contain data for “Lipid_T=XC_Yfps_frameZ” (e.g. DMPC_T=6C_frame1), where “Lipid” is the lipid type or mixture, “X” is the temperature of the sample, “Y” is the number of frames per second images were acquired at, and “Z” is the frame number. Supplementary Fig 3 – “Lipid_TcX_ThY_z_zh=Z” (e.g. DOPC_Tc25_Th55_z_zh=0.00) where “Lipid” is the lipid type, “X” is the temperature of the cold plate in ˚C, “Y” is the temperature of the hot plate in ˚C, and “Z” refers to the height fraction z/zh above the cold plate i.e. z_zh=0.00 would be the cold plate. Supplementary Fig 4 – “Lipid_pH_X_TbarY_runZ_sliceA” (e.g. DOPC_pH_5_5_Tbar30_run1_slice1) where “Lipid” is the lipid type, “X” is the pH e.g. 5_5 is pH 5.5, “Y” is the mean temperature between the hot and cold plates e.g. 10 refers to 10˚C, “Z” is the run number (between 1 and 5), “A” is the slice number where slice 1 is at the cold plate and slice 19 at the hot plate. Alternatively “Lipid_chol_TbarY_runZ_sliceA” for membranes containing chol. Supplementary Fig 5 – Size distribution data are contained in folders specific to the lipid type with images “Lipid_frameX” where “X” refers to the frame number and “Lipid” the lipid type e.g. DGDG. Images were acquired at 10fps at a temperature of 40 ˚C. Supplementary Fig 6 – “Lipid_TcX_ThY_z=Zum_frameA” (e.g. DGDG_Tc5_Th15_z=8um_frame1) where “Lipid” is the lipid type, “X” is the temperature of the cold plate in ˚C, “Y” is the temperature of the hot plate in ˚C, “Z” is the distance in µm above the cold plate, and “A” is the frame number. Frames were acquired at 10fps. This record is pending publication in Nature communications ("Thermophoretic Migration of Vesicles Depends on Mean Temperature and Head Group Chemistry. Experimental dataset."). This record is embargoed until publication and it will be updated.

本数据集包含发表于《Nature Communications》的论文《囊泡（vesicle）的热泳迁移（thermophoretic migration）依赖于平均温度与头部基团化学性质》的配套数据，作者为Emma L. Talbot、Jurij Kotar、Lucia Parolini、Lorenzo Di Michele与Pietro Cicuta，DOI：10.1038/NCOMMS15351。本数据集收录了囊泡在热梯度中运动相关的原始图像。每个压缩文件夹内存储了对应正文中或补充材料中引用该数据的插图的全分辨率TIFF（.tiff）格式荧光显微成像（fluorescence microscopy）数据图像。每个像素对应0.145微米（μm）。荧光测量与zeta电位（zeta-potential）测量（对应图5与补充图9）的数据以Excel电子表格形式提供。详细信息请参阅原文。
各图像命名规则如下，对应正文中的插图：
1. 正文图1c：命名格式为`State_z_zh=X`（例如`Initial_z_zh=0.05`），其中`State`可取`initial`（初始态）或`steady state`（稳态），分别指代数据记录于t=0 min时或达到稳态后；`X`代表冷板（cold plate）上方的高度分数z/zh，即`z_zh=0.00`对应冷板位置。
2. 正文图1d与补充图8：命名格式为`DiPhyPC_TcX_ThY_dT=Z`（例如`DiPhyPC_Tc15_Th45_dT=3.0K`），其中`DiPhyPC`为脂质（lipid）类型，`X`为冷板温度（单位℃），`Y`为热板（hot plate）温度（单位℃），`Z`为相对于冷板的温差。
3. 正文图3：命名格式为`Lipid_Tbar_X_runY_sliceZ`（例如`DGDG_Tbar_10_run1_slice1`），其中`Lipid`为脂质类型（涵盖DGDG、SQDG、DiPhyPC、DPPC、DMPC、DOPC、DOPG及POPG），`X`为冷热板之间的平均温度（例如`10`代表10℃），`Y`为实验批次号（run number，取值范围1至5），`Z`为切片编号（slice number），切片1对应冷板位置，切片19对应热板位置。
4. 正文图4：文件夹内数据命名格式为`Lipid_T=XC_frameY`（例如`DMPC_T=6C_frame1`），其中`Lipid`为脂质类型或混合物，`X`为样品温度，`Y`为帧编号。图像以10fps（帧每秒）采集。
5. 正文图6与补充图7：命名格式为`Lipid_TcX_ThY_t=Zmin_sliceA`（例如`DOPC_Tc25_Th55_t=0min_slice1`），其中`Lipid`为脂质类型，`X`为冷板温度（℃），`Y`为热板温度（℃），`Z`为采集时间（单位min），`A`为切片编号，切片1对应冷板，切片19对应热板。
6. 正文图7：命名格式为`Lipid1_Lipid2_Tbar_X_runY_sliceZ`（例如`DOPC_DOPG_Tbar_15_run1_slice1`），其中`Lipid1`为第一种脂质类型，`Lipid2`为第二种脂质类型，对应脂质1/脂质2/胆固醇的囊泡体系；`X`为冷热板平均温度，`Y`为批次号（1至5），`Z`为切片编号（切片1为冷板，19为热板）。
7. 正文图8：文件夹内数据命名格式为`Lipid_T=XC_Yfps_frameZ`（例如`DMPC_T=6C_frame1`），其中`Lipid`为脂质类型或混合物，`X`为样品温度，`Y`为图像采集帧率，`Z`为帧编号。
针对补充插图的命名规则：
1. 补充图3：命名格式为`Lipid_TcX_ThY_z_zh=Z`（例如`DOPC_Tc25_Th55_z_zh=0.00`），其中`Lipid`为脂质类型，`X`为冷板温度（℃），`Y`为热板温度（℃），`Z`为冷板上方的高度分数z/zh，`z_zh=0.00`对应冷板位置。
2. 补充图4：命名格式为`Lipid_pH_X_TbarY_runZ_sliceA`（例如`DOPC_pH_5_5_Tbar30_run1_slice1`），其中`Lipid`为脂质类型，`X`为pH值（例如`5_5`代表pH 5.5），`Y`为冷热板平均温度，`Z`为批次号（1至5），`A`为切片编号（切片1对应冷板，19对应热板）。对于含胆固醇的膜体系，命名格式为`Lipid_chol_TbarY_runZ_sliceA`。
3. 补充图5：尺寸分布数据存储于对应脂质类型的专属文件夹中，图像命名为`Lipid_frameX`，其中`X`为帧编号，`Lipid`为脂质类型（例如`DGDG`）。图像于40℃下以10fps采集。
4. 补充图6：命名格式为`Lipid_TcX_ThY_z=Zum_frameA`（例如`DGDG_Tc5_Th15_z=8um_frame1`），其中`Lipid`为脂质类型，`X`为冷板温度（℃），`Y`为热板温度（℃），`Z`为冷板上方的距离（单位μm），`A`为帧编号。图像以10fps采集。
本数据集即将发表于《Nature Communications》的文章"Thermophoretic Migration of Vesicles Depends on Mean Temperature and Head Group Chemistry. Experimental dataset."。本数据集在正式发表前处于保密期，后续将进行更新。