Data from: Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Attached file provides supplementary data for linked article.

Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.

本附件文件为关联文章提供补充数据集。炭疽病是普通菜豆（Phaseolus vulgaris L.）的一种毁灭性病害。研究证实，安第斯菜豆品种红云豆对炭疽病菌生理小种81具有较强抗性。为解析该抗性的遗传机制，本研究将红云豆与感病基因型品种京豆进行杂交，并在可控环境条件下，针对F₂群体及F₂:₃家系对生理小种81的抗性分离情况开展评估。结果表明，红云豆携带1个显性单基因调控其对炭疽病的抗性。通过将红云豆与另一抗病品种杂交进行等位性测验，发现该抗性基因位于Co-1基因座（故将其命名为Co-1HY）。该基因座与两侧翼标记之间的物理距离为46 kb，此区域包含4个候选基因，分别为Phvul.001G243500、Phvul.001G243600、Phvul.001G243700及Phvul.001G243800。上述候选基因均编码丝氨酸/苏氨酸蛋白激酶。本研究对4个候选基因在抗病与感病品种中分别于对照条件及接种处理下的表达水平进行分析，结果显示所有候选基因在抗病基因型中的表达量均显著高于感病基因型。接种前，Phvul.001G243600与Phvul.001G243700在抗病基因型中的表达量分别较感病亲本高出近15倍与90倍。上述4个候选基因为后续解析普通菜豆抗炭疽病机制的相关研究提供了重要参考。本研究鉴定得到的紧密连锁侧翼标记，可用于将红云豆中的抗性等位基因Co-1HY转入优良感炭疽病普通菜豆品系中。