Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line

Vitis vinifera (black raisin) is commonly used in traditional medicine for the treatment of various ailments. In the present study, anti-oxidative and anti-cancer efficacy of oleanolic acid from ethyl acetate fraction of black raisins was evaluated and oleanolic acid was isolated without using of any chromatographic techniques and subjected to spectral assessment using UV-Vis spectrophotometer, 1H NMR, 13C NMR, MS and FT-IR for structural confirmation. Antiproliferative efficacy of oleanolic acid against human colon adenocarcinoma HCT-116 cells was assessed using cell viability assay. The minimum inhibitory concentration (IC50) was determined and found to be 40 µg/mL at 48h incubation. Furthermore, antioxidant property of oleanolic acid was analyzed using DPPH method (IC50 is 61.5µg/mL) by compared to standard antioxidants ascorbic acid, gallic acid, pyrogallol and butylated hydroxytoluene. Hence, the present study aims to establish the use of oleanolic acid as a potential therapeutic agent against human colon cancer.

黑葡萄干（Vitis vinifera）在传统医学中常被用于治疗多种疾病。本研究对黑葡萄干乙酸乙酯萃取组分中的齐墩果酸（oleanolic acid）的抗氧化与抗癌活性进行了评价；本研究未借助任何色谱技术分离得到齐墩果酸，并通过紫外-可见分光光度计（UV-Vis spectrophotometer）、氢核磁共振谱（¹H NMR）、碳核磁共振谱（¹³C NMR）、质谱（MS）及傅里叶变换红外光谱（FT-IR）进行光谱表征以确认其结构。本研究采用细胞活力检测法，评估了齐墩果酸对人类结肠腺癌细胞HCT-116的抗增殖活性。经测定，孵育48小时后的半数抑制浓度（IC50）为40 µg/mL。此外，本研究采用DPPH自由基清除法，以抗坏血酸、没食子酸、焦没食子酚及二叔丁基对甲酚为阳性对照，分析了齐墩果酸的抗氧化活性，其IC50为61.5 µg/mL。综上，本研究旨在确立齐墩果酸作为抗人类结肠癌潜在治疗剂的应用价值。