CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [CRISPR_set8]

In this experiment, we sought to analyze the genes positively or negatively selected during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) with particular focus on the genes enriched for CTCF boundary disruption at HoxA cluster Examination of the genes positively or negatively selected in embryonic stem cells and cervical motor neurons using CRISPR loss-of-function screens, with particular focus on the genes enriched for CTCF boundary disruption at HoxA cluster