FoxO3 prevents premature termination of liver regeneration by suppressing hepatocyte proliferation

The liver is a typical organ that is capable of substantial regeneration upon injury in adult mammals. To data, the 70% partial hepatectomy (PH) represents the most commonly used model for the study of liver regeneration . Despite the remove of 2/3 mass, liver can fully regrow from the remaining mass through mitosis of hepatocytes. After PH, hepatocytes are the first liver cells to re-enter the cell cycle and undergo 1-2 rounds of cell division until the original liver mass is restored.The transcriptional factor FoxO3 is pivotal to a various of liver diseases but its role in liver regeneration remains unclear. For total RNA isolation, liver tissues were extracted in sham and injured mice at 4 days post-PH (dpH),respectively. Three mice per group were used for RNA-sequencing analysis. RNA preparation, library construction and sequencing on GBISEQ-500 platform was performed as previously described.