Functional enzyme analysis and metabolic regulation mechanism of the combined microflora LXB in the degradation of lignocellulose

The biodegradation of lignocellulosic biomass is a practical and effective method. A random combination of six isolates and two purchased strains was constructed to obtain LXB, the degradation of lignocellulose resulted in a 40.6% after 168 hours. A total of 1872 genes that were differentially expressed and involved in the degradation of lignocellulose in LXB have been identified in the Carbohydrate-Active enZYmes Database. Genes that encode cellulases and hemicellulase were expressed in abundance, the gene that encodes hemicellulase displaying highest expression at the initial and peak stages of degradation. Considerable increase in gene expression of Versatile peroxidase and coenzyme Alcohol oxidase involved in lignin catabolism. LXB catalyzes the breakdown of lignocellulose mainly through six pathways in the carbohydrate metabolic pathway, catechol was discovered in the benzoate degradation pathway, which participates in the degradation of lignin. Additionally, LXB possesses the capability to degrade harmful exogenous substances.