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Functional Changes of CD8+ T Cells in Tumor Microenvironment after SIGLEC15 Blockade [bulk RNA-seq]. Functional Changes of CD8+ T Cells in Tumor Microenvironment after SIGLEC15 Blockade [bulk RNA-seq]

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA903800
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To understand how SIGLEC15 regulates CD8+ T cells in tumor microenvironment, we enriched CD8+ T cells using magnetic beads after tumor digestion. Collected CD8+ T cells were examined with flowcytometry analysis and were further loaded for sequencing analysis. Overall design: IgG (n=6) and anti-SIGLEC15 (n=6) treated percutaneous tumor grafts were constructed in C57 mice and collected in day19 after 2 weeks of intraperitoneal injection. Collected CD8+ T cells were stained for further examination in flowcytometry analysis. After confirmation, 3 samples of collected cell pelletes were mixed together and loaded for sequencing analysis.
创建时间:
2022-11-21
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