five

Quantification of synthetic communities

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NIAID Data Ecosystem2026-05-02 收录
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http://flowrepository.org/id/FR-FCM-Z8YN
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Determine the community composition of oral bacterial synthetic communities through flow cytometry and PCR-based methods. Conclusion: Flow cytometry is a good tool to determine the community composition of mock communities. However, in current format, it is not suitable for the determination of co-cultures because of changing phenotypes while co-culturing bacteria. Daily QC of flow cytometer - Biological triplicates - Axenic control samples - Medium blancs - Dilutant blancs - Singlet analysis - Stability fluorescent signal over time
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2024-08-01
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