Protein Quantification and Identification from River Bank Grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.

The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with concurrent label-free quantitation in order to optimise a technique that would facilitate identification of most number of proteins and produce useful biological information, when searched against the Vitis vinifera database.