MicroRNA sequence and expression analysis of rat liver tissue exposed to aflatoxin B1 by deep sequencing

We report the application of illumina deep sequencing technology for high-throughput profiling of microRNA in aflatoxin B1 treated rat liver tissue, as well as normal liver tissue. miRNAs of miR-17-92 cluster, thought to be tumor activating miRNAs, were down-regulated. miR-34a, considered to be a tumor suppressor, was up-regulated.Novel miRNAs and miRNA base alteration caused by SNP or editing were predicted and summarized.本研究采用Illumina深度测序技术，对经黄曲霉毒素B1（aflatoxin B1）处理的大鼠肝组织与正常大鼠肝组织中的微小RNA（microRNA, miRNA）开展高通量图谱分析。研究发现，被认为具有促肿瘤活性的miR-17-92簇微小RNA的表达水平显著下调；而被视作肿瘤抑制因子的miR-34a，其表达水平则明显上调。此外，本研究还对由单核苷酸多态性（SNP）或RNA编辑诱导产生的新型微小RNA以及微小RNA碱基变异进行了预测与汇总分析。