Analysis of mutations in the fabI gene of S. epidermidis and the upstream promoter region.

The suffix a* indicates adapted strains that have been passed for 5 days on triclosan free media. The fabI primers did not work on three isolates (65-13, BD-26 and BD-50). S. epidermidis ATCC 12228 is published on NCBI with its full sequence. It has a triclosan MIC fabI gene is identical to all the current triclosan susceptible isolates we have sequenced. The putative promoter region of ATCC 12228 is different from the current triclosan susceptible isolates we have sequenced but those are all identical. ST, Sequence type determined by MLST. ND = not determined. Abbreviations for amino acids: H = histidine, Y = tyrosine, S = serine, A = alanine, G = glycine, F = phenylalanine, V = valine, Q = glutamine.