Summary statistics of six libraries generated with the presented cost-conscious protocol and sequenced on the Illumina NovaSeq 6000 platform.

Libraries consist of four plants (Acacia acuminata, Angohpora floribunda, Corymbia maculata, Eucalyptus melliodora) and two fungi (Puccinia striiformis f. sp. tritici, Puccinia triticina). Quality scores are presented based on the Phred scale. PCR duplicates were calculated independent of a reference genome, with HTStream SuperDeduper [17]. Expected coverage was estimated by sequencing output divided by genome size. Observed coverage was calculated by mapping the reads against an independently generated long-read de novo assembly. SD denotes standard deviation.