Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 50 cells per microliter

A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. The cells were mixed at a final concentration of 50 cells per microliter. Overall design: An estimated 1000 STAMPs and 100 STAMPs were separately selected for sequencing to obtain both high coverage and broad coverage data

本实验采用HEK细胞与3T3细胞等比例混合制备的细胞悬液，应用于Drop-Seq——一种用于高通量单细胞mRNA测序（single cell mRNAseq）的新型技术。细胞混合后的终浓度为每微升50个细胞。实验整体设计：分别选取约1000个STAMPs与100个STAMPs进行测序，以同时获取高覆盖度与广覆盖度的测序数据。