Crystal structure of a Mn(II)-based complex and application of its nanoparticles for treatment activity on peri-implantitis by reducing inflammatory responses

A novel coordination polymer (CP), namely, [Mn(tib)₂(NO₃)₂]_n (<b>1</b>, tib = 1,3,5-tri(1H-imidazol-1-yl)benzene) has been prepared under hydrothermal condition and characterized by elemental analyses, infrared spectroscopy and single-crystal X-ray diffraction analyses. Furthermore, a green hand grinding technique has been implemented to reduce the particle size of the complex <b>1</b> to generate nanoscale <b>1</b> (denoted as nano 1 hereafter). In addition, its protective effect on the peri-implantitis diseases was determined. The peri-implantitis animal model was established and the compound was given for indicated treatment. The macrophages polarization in the periodontal tissue was detected with flow cytometry. Then, Enzyme Linked Immune Sorbent Assay (ELISA) detection kit was performed to measure the Interleukin-1β (IL-1β) and Tumor Necrosis Factor-α (TNF-α) content in the tissues after compound treatment.

一种新型配位聚合物（coordination polymer, CP），化学式为[Mn(tib)₂(NO₃)₂]ₙ（标记为**1**，其中tib为1,3,5-三(1H-咪唑-1-基)苯），在水热条件下制备得到，并通过元素分析、红外光谱及单晶X射线衍射进行了表征。进一步采用绿色手工研磨技术对配合物**1**进行粒径缩减，得到纳米级配合物**1**（下文简称纳米**1**）。此外，本研究评估了该化合物对种植体周围炎的防护效果：构建种植体周围炎动物模型，按指定方案给予该化合物进行治疗；通过流式细胞术检测牙周组织中的巨噬细胞极化情况；采用酶联免疫吸附测定（Enzyme Linked Immune Sorbent Assay, ELISA）试剂盒检测给药后组织中白细胞介素-1β（IL-1β）与肿瘤坏死因子-α（TNF-α）的含量。