Whole Genome Bisulfite sequencing for liver DNA methylation analysis of rat exposed to 0.05% Pb

We generated 4 Gb of high-quality sequencing data (~1 Gb per sample). The NCD+0.05%Pb group had hypermethylation at the whole genome level, compared to NCD. Difference analysis in gene methylation aimed at TSS region indicated that a total of 2733 genes experienced DNA methylation changes, with 2320 hypermethylated genes and 413 hypomethylated genes of the NCD+0.05%Pb group compared with the NCD group.本研究生成了4 Gb的高质量测序数据，单样本测序数据量约为1 Gb。与NCD组相比，NCD+0.05%Pb组在全基因组水平呈现高甲基化特征。针对转录起始位点（Transcription Start Site, TSS）区域开展基因甲基化差异分析后发现，相较于NCD组，NCD+0.05%Pb组共有2733个基因发生DNA甲基化改变，其中高甲基化基因2320个，低甲基化基因413个。